LOCUS       Exported                4454 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0097_pluxRRR12y-sfGFP-stRBS-luxI
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="R2oDNA spacer"
                     /note="R2oDNA 70bp spacer 13"
     terminator      203..230
                     /note="T7Te"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(238..308)
                     /note="taR12y"
     promoter        complement(309..363)
                     /note="lux rR (R0062)"
     misc_feature    364..433
                     /note="spacer (B0040)"
     promoter        434..488
                     /note="lux rR (R0062)"
     3'UTR           489..540
                     /note="crR12y"
     RBS             523..534
                     /note="RBS (B0034)"
     CDS             541..1266
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     RBS             1275..1286
                     /note="RBS (B0034)"
     CDS             1293..1877
                     /codon_start=1
                     /note="luxI (C0161)"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*"
     terminator      1889..1975
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1969..1996
                     /note="T1"
     primer_bind     2003..2022
                     /note="AB179"
     primer_bind     2188..2207
                     /note="AB5"
     rep_origin      2341..3168
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      2589..2591
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(2592..3136)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     3240..3259
                     /note="AB180"
     oriT            3270..3378
                     /note="incP origin of transfer"
     misc_feature    3350
                     /note="C->T no real mutation, just for testing"
     CDS             complement(3534..4349)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg
      181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct
      241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg
      301 atttagcttt tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca
      361 ggtaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt
      421 cactgaccta acaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt
      481 cgaataaaga attctaccat tcacctcttg gatttagcta ttaaagagga gaaaggtacc
      541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
      661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
      781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
      961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct
     1261 taataatact agtgaaagag gagaaatact agatgactat aatgataaaa aaatcggatt
     1321 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta
     1381 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata
     1441 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc
     1501 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc
     1561 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa
     1621 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat
     1681 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag
     1741 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg
     1801 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag
     1861 cagtcttaaa ttaataaatt aaaggcatca aataaaacga aaggctcagt cgaaagactg
     1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     1981 gccctagaca gctgggcgcg ccaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2041 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
     2101 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
     2161 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
     2221 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
     2281 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
     2341 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
     2401 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
     2461 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     2521 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     2581 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     2641 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct
     2701 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2761 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     2821 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     2881 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     2941 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     3001 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     3061 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     3121 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agcatgagcg
     3181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3241 gaaaagtgcc acctgctagg gccggcctac ggccagcctc gcagagcagg attcccgttg
     3301 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgt gggtgggcct
     3361 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     3421 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     3481 atgaccccga agcagggtta tgcagcggaa aaggaccctt gtccaattaa ttattagaaa
     3541 aattcatcca gcatcagatg aaattgcagt ttgttcatat ccggattatc aatgccatat
     3601 ttctgaaaca gacgtttttg caggctcggg ctaaattcgc ccaggcagtt ccacagaatg
     3661 gccagatcct gataacgatc cgcaatgccc acacggccca catcaatgca gccaatcagt
     3721 ttgccttcat cgaaaatcag gttatccagg ctaaaatcgc cgtgggtcac cacgctatcc
     3781 gggctaaacg gcagcagttt atgcatttct ttccacacct gttccaccgg ccagccgtta
     3841 cgttcatcat caaaatcgct cgcatccacc aggccgttgt tcatacggct ctgcgcctgg
     3901 gccagacgaa acacacgatc gctgttaaac gggcagttgc acaccggaat gctatgcaga
     3961 cgacgcagaa acacggccag cgcatccaca atgttttcgc cgctatccgg atattcttcc
     4021 agcacctgaa acgcggtttt gcccggaatc gcggtggtca gcagccacgc atcatccggg
     4081 gtgcgaataa aatgtttaat ggtcggcagc ggcataaatt cggtcagcca gttcagacgc
     4141 accatttcat cggtcacatc gttcgccacg ctgcctttgc catgtttcag aaacagttcc
     4201 ggcgcatccg gtttgccata cagacgataa atggtcgcgc cgctctgacc cacgttatca
     4261 cgcgcccatt tatagccata cagatccgca tccatgttgc tgttcagacg cggacggcta
     4321 cagctcgttt cacgctgaat atggctcata acaccccttg tattactgtt tatgtaagca
     4381 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     4441 tgagacacaa attt
//
